ABSTRACT The present study was conducted to investigate the efficacy of oral administration of L. reuteri on growth performance, intestine histomorphology, immunological and gut microbiome of broilers. A total of twenty healthy chickens were used in a five-week experimental trial. Birds were assigned into one of two groups with orally administrated L. reuteri probiotic and without probiotic- (Control -Phosphate-buffered saline). A significant (p<0.05) body weight gain was observed in the chickens in L. reuteri treatment group compare to those in the control group at the end of the trial. In addition, the serum IGF-1 cytokines level significantly enhanced in L. reuteri treatment group. However, there were no notable effects observed on the villus height, crypt depth, muscularis thickness, and submucosal thickness in chickens orally inject with and without L. reuteri. At the phylum level, the presence of Firmicutes (99.5%) was highly abundantin the L. reuteri treatment group. Moreover, the fecal microbial communities of Lactobacillus (99.9%) showed average relative abundance at genus level in L. reuteri treatment group. From this, we concluded that oral administration of L. reuteri would be beneficial to enhance the body weight gain, gut microbiome, and immune status of broiler.

ABSTRACT A total of 792 conventional healthy 1-day-old Ross 308 broilers chicks (mixed gender) with average body weight of 42.30±1.14 g (mean ± SD) were used in the experiment, which lasted for 35 days. Chicks were randomly allotted into one of four treatment diets, each one having 11 replicate cages with 18 birds each, being fed corn-soybean meal (SBM) based diets. Dietary treatments were CON (basal diet with unvaccinated birds); OA = CON + 0.1% organic acids; ACB = CON + anti-coccidium vaccine; OAACV = CON + 0.1% organic acid + anti-coccidium vaccine. Significant results were observed, with improved body weight (p=0.059; 0.064; 0.034) during days 1-7, 8-21, and overall, respectively. Significant effects were also observed on the feed conversion ratio (p=0.037) through the overall experiment, with no effects on feed intake on OAACV as compared to other treatment groups. Total track digestibility of dry matter (p=0.049) improved significantly in the OAACV treatment group. Additionally, beneficial effects were observed in the OAACV treatment group, with improvements in fecal microbial population (increased lactobacillus) and reduced NH3 gas emissions. Broilers fed the OAACV treatment tended to display reduced drip loss in the meat samples analyzed on days 5 and 7 (p=0.067, 0.072). In summary, our findings revealed that dietary inclusion of organic acid supplementation with anti-coccidium had a beneficial effect on broilers affected by coccidian infection, also improving growth performance, digestibility, fecal Lactobacillus counts, and reducing NH3.

Resection and hand-sewn Billroth I reconstruction is the commonly carried out procedure for type I gastric strictures. The use of staplers for Billroth I anastomosis is limited. Here we describe a unique technique of Billroth I anastomosis using a circular stapler without a gastrotomy in a case of corrosive gastric stricture. A fourteen-year-old girl with Type I gastric stricture underwent resection of the pre-pyloric stricture and Billroth I reconstruction, for which the shaft of the circular stapler was inserted through the open end of the stomach and a side-to-end anastomosis of the posterior wall of the stomach with the end of the duodenum was carried out. The distal cut open end of the stomach was closed with a linear cutter. The patient made an uneventful recovery. A circular stapler can be used by this technique for Billroth I anastomosis for a pre-pyloric corrosive stricture without a need for additional gastrotomy for introducing the stapler.

INTRODUCTION: Resection and hand-sewn Billroth I reconstruction is the commonly carried out procedure for type I gastric strictures. The use of staplers for Billroth I anastomosis is limited. Here we describe a unique technique of Billroth I anastomosis using a circular stapler without a gastrotomy in a case of corrosive gastric stricture. CASE SUMMARY AND TECHNIQUE: A fourteen-year-old girl with Type I gastric stricture underwent resection of the pre-pyloric stricture and Billroth I reconstruction, for which the shaft of the circular stapler was inserted through the open end of the stomach and a side-to-end anastomosis of the posterior wall of the stomach with the end of the duodenum was carried out. The distal cut open end of the stomach was closed with a linear cutter. The patient made an uneventful recovery. CONCLUSION: A circular stapler can be used by this technique for Billroth I anastomosis for a pre-pyloric corrosive stricture without a need for additional gastrotomy for introducing the stapler.